Nkx2-2^tm2.1Suss
Targeted Allele Detail

Summary

• Symbol: Nkx2-2^tm2.1Suss
• Name: NK2 homeobox 2; targeted mutation 2.1, Lori Sussel
• MGI ID: MGI:5297810
• Synonyms: Nkx2.2^TNmut
• Gene: Nkx2-2 Location: Chr2:147019466-147036163 bp, - strand Genetic Position: Chr2, 72.63 cM, cytoband H
• Alliance: Nkx2-2^tm2.1Suss page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177838
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (RMCE-ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Recombination mediated cassette exchange (RMCE) into Nkx2-2^tm1Suss inserted nucleotide substitutions that result in several amino acid substitutions in the tinman (TN) domain (TGFSVKDILDLP to TGFAAAAIVDLP). An FRT-flanked neo cassette used for selection was removed by flp-mediated recombination. (J:177838)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nkx2-2 Mutation: 14 strains or lines available

References

• Original: J:177838 Papizan JB, et al., Nkx2.2 repressor complex regulates islet beta-cell specification and prevents beta-to-alpha-cell reprogramming. Genes Dev. 2011 Nov 1;25(21):2291-305
• All: 2 reference(s)