Tg(RNU6-RNAi:Mpp5)13Wij
Transgene Detail

Summary

• Symbol: Tg(RNU6-RNAi:Mpp5)13Wij
• Name: transgene insertion 13, Jan Wijnholds
• MGI ID: MGI:5297927
• Synonyms: shPals1^flox
• Transgene: Tg(RNU6-RNAi:Mpp5)13Wij Location: unknown
• Alliance: Tg(RNU6-RNAi:Mpp5)13Wij page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:178056
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Transgene description

• Transgene Type: Transgenic (Knockdown)
• Mutation: Insertion
• Tg(RNU6-RNAi:Mpp5)13Wij involves 1 genes/genome features (Pals1) View all
• Mutation details: The trasngene contains the human RNU6 promoter driving expression of a short-hairpin RNA targeting Mpp5 preceded by a floxed puro cassette and followed by the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) sequences. ES cell lines 7 and 13 generated germ line transmission. (J:178056)

Expression

In Structures Affected by this Mutation:

14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:178056 Park B, et al., PALS1 Is Essential for Retinal Pigment Epithelium Structure and Neural Retina Stratification. J Neurosci. 2011 Nov 23;31(47):17230-17241
• All: 2 reference(s)