Dppa2^tm1.2Yam
Targeted Allele Detail

Summary

• Symbol: Dppa2^tm1.2Yam
• Name: developmental pluripotency associated 2; targeted mutation 1.2, Shinya Yamanaka
• MGI ID: MGI:5298642
• Synonyms: 15-2^FRT
• Gene: Dppa2 Location: Chr16:48130637-48139876 bp, + strand Genetic Position: Chr16, 30.75 cM
• Alliance: Dppa2^tm1.2Yam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:178324
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: An FRT site was inserted into intron 1 and an FRT flanked hyg cassette site was inserted after the last exon via homologous recombination. This allele was originally created in tandem with Dppa4^tm1Yam. These alleles are in cis to each other. Flp mediated recombination removed the hyg cassette. Elevated transcript expression, but not protein expression, was detected in the testes of mice heterozygous for this allele and Dppa4^tm1.1Yam. (J:178324)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dppa2 Mutation: 27 strains or lines available

References

• Original: J:178324 Nakamura T, et al., Essential roles of ECAT15-2/Dppa2 in functional lung development. Mol Cell Biol. 2011 Nov;31(21):4366-78
• All: 1 reference(s)