Emc4^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Emc4^{tm1(KOMP)Wtsi}
• Name: ER membrane protein complex subunit 4; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5299564
• Gene: Emc4 Location: Chr2:112193356-112198372 bp, - strand Genetic Position: Chr2, 57.02 cM, cytoband E4
• Alliance: Emc4^{tm1(KOMP)Wtsi} page
• IMPC: Emc4 gene page

Mutation origin

• Mutant Cell Lines: EPD0888_3_A01, EPD0888_3_A03, EPD0888_3_B01, EPD0888_3_B04, EPD0888_3_D01, EPD0888_3_D03, EPD0888_3_E02, EPD0888_3_G01, EPD0888_3_G03, EPD0888_3_G04, EPD0888_3_H01, EPD0888_3_H02
• Germline Transmission: Earliest citation of germline transmission: J:211773
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 3802 starting at position 112194080 and ending at position 112197882 of Chromosome 2 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Emc4 Mutation: 7 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 4 reference(s)