TgTn(sb-Tyr)1799BOve
Transgene Detail

Summary

• Symbol: TgTn(sb-Tyr)1799BOve
• Name: transgenic transposon concatemer 1799B, Paul A Overbeek
• MGI ID: MGI:5300386
• Transgene: TgTn(sb-Tyr)1799BOve Location: unknown
• Alliance: TgTn(sb-Tyr)1799BOve page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Transposon concatemer)
• Mutation: Insertion
• Mutation details: A Sleeping Beauty (SB) transposon transgenic approach was used to generate these mice. The pT2-Tyro-Gal4-SV40 transposon vector used here has an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site [280 bp]), a Gal4-VP16-SV40pA sequence (1.7 kbp), the Tyro minigene (4.1 kbp), and right IR/DR (280 bp). The IR/DR sequences are outward-facing (pointed away from the Gal4-VP16-SV40pA::Tyro sequences). The Gal4-VP16-SV40 cassette is in the opposite orientation to the Tyro minigene. The 4.1 kb tyrosinase minigene (Tyro) was isolated from plasmid Ty811C and contains the tyrosinase upstream regulatory sequences (2.25 kb from the BALB/c tyrosinase promoter and the first 65 bp of exon 1) and a chimeric cDNA made with (from 5' to 3') the upstream part of the C57BL/6-derived Tyrs-J (cysteine at amino acid 103) sequence ligated at a central ScaI site to the downstream part of the DBA/2-derived tyrosinase (valine at amino acid 346). Line 1799B contains 15 copies of the transposon on chromosome 18 (18B3; 76,860,053 [from NCBI37/mm9 (July 2007)). (J:178818)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:178818 Wang B, et al., Transposon mutagenesis with coat color genotyping identifies an essential role for Skor2 in sonic hedgehog signaling and cerebellum development. Development. 2011 Oct;138(20):4487-97
• All: 1 reference(s)