Kitl^tm1.1Sjm
Targeted Allele Detail

Summary

• Symbol: Kitl^tm1.1Sjm
• Name: kit ligand; targeted mutation 1.1, Sean J Morrison
• MGI ID: MGI:5300819
• Synonyms: Scf^gfp
• Gene: Kitl Location: Chr10:99851492-99936278 bp, + strand Genetic Position: Chr10, 51.4 cM
• Alliance: Kitl^tm1.1Sjm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:180431
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exon 2 with EGFP (in frame) and an FRT-flanked neomycin resistance cassette. The neomycin resistance cassette was removed by flp-mediated recombination. qRT-PCR confirmed the absence of transcript expression in the liver. (J:180431)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kitl Mutation: 94 strains or lines available

References

• Original: J:180431 Ding L, et al., Endothelial and perivascular cells maintain haematopoietic stem cells. Nature. 2012 Jan 26;481(7382):457-62
• All: 19 reference(s)