Nkx3-2^{tm4.1(cre)Tlu}
Targeted Allele Detail

Summary

• Symbol: Nkx3-2^{tm4.1(cre)Tlu}
• Name: NK3 homeobox 2; targeted mutation 4.1, Thomas Lufkin
• MGI ID: MGI:5300901
• Synonyms: Bapx1^FCFE
• Gene: Nkx3-2 Location: Chr5:41918826-41921563 bp, - strand Genetic Position: Chr5, 22.58 cM
• Alliance: Nkx3-2^{tm4.1(cre)Tlu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:178893
• Parent Cell Line: v6.4 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Nkx3-2^{tm4.1(cre)Tlu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nkx3-2 (MGI:108015)

• Mutation details: A targeting vector was generated by inserting an F2A-cre-F2A-EGFP-Frt-PGK-gb2-Neo-Frt cassette immediately before the stop codon of of the Nkx3-2 gene sequence in a BAC (RP24-148P5). The 19.6 kb BAC targeting vector was electroporated into V6.4 ES cells for homologous recombination. The neo cassette was subsequently removed by FLPe-mediated recombination. (J:178893)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nkx3-2 (mouse) Summary of all recombinase alleles driven by Nkx3-2. Comparative matrix view of recombinase activities Alliance mouse Nkx3-2 gene page

Expression

In Mice Carrying this Mutation:

7 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nkx3-2 Mutation: 21 strains or lines available

References

• Original: J:178893 Sivakamasundari V, et al., New Bapx1(Cre-EGFP) mouse lines for lineage tracing and conditional knockout studies. Genesis. 2012 Apr;50(4):375-83
• All: 2 reference(s)