Pparg^{tm1.1(tTA)Jmgr}
Targeted Allele Detail

Summary

• Symbol: Pparg^{tm1.1(tTA)Jmgr}
• Name: peroxisome proliferator activated receptor gamma; targeted mutation 1.1, Jonathan M Graff
• MGI ID: MGI:5300919
• Synonyms: PPARgamma-tTA
• Gene: Pparg Location: Chr6:115337912-115467360 bp, + strand Genetic Position: Chr6, 53.41 cM, cytoband E3-F1
• Alliance: Pparg^{tm1.1(tTA)Jmgr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:178521
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Transactivator)
• Mutation: Insertion
• Mutation details: A targeting construct was electroporated into ES cells and by homologous recombination, a tTA-Frt-neo-Frt (tTA = tetracycline transactivator) sequence was inserted into the second coding exon of Pparg, creating an in-frame fusion between the amino terminus of Pparg and tTA. Correctly targeted ES cells were used to generate mice. The neo resistance cassette was removed from the germline by crossing Pparg mutants with FLPe-expressing mice. (J:178521, J:212531)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pparg Mutation: 41 strains or lines available

Notes

ES cell line = ZX1, a mutant A17 line carrying an inducible cassette exchange allele at Hprt. The A17 ES cell line used to make the ZX1 cells is a mutant E14TG2A parental ES cell line carrying an inserted rtTA sequence at the Rosa26 locus.

References

• Original: J:178521 Tang W, et al., White fat progenitor cells reside in the adipose vasculature. Science. 2008 Oct 24;322(5901):583-6
• All: 15 reference(s)