Nos1^tm1(cre)Mgmj
Targeted Allele Detail

Summary

• Symbol: Nos1^tm1(cre)Mgmj
• Name: nitric oxide synthase 1, neuronal; targeted mutation 1, Martin G Myers, Jr
• MGI ID: MGI:5301174
• Synonyms: Nos1^cre, Nos1-ires-cre
• Gene: Nos1 Location: Chr5:118004904-118096905 bp, + strand Genetic Position: Chr5, 57.29 cM, cytoband F
• Alliance: Nos1^tm1(cre)Mgmj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:179120
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Nos1^tm1(cre)Mgmj expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nos1 (MGI:97360)

• Mutation details: An IRES-cre-Frt-neo-Frt cassette was inserted after the stop codon of the final 3' exon of the Nos1 gene by homologous recombination in R1 ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to C57BL/6J for 9 generations. (J:179120, J:183754)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nos1 (mouse) Summary of all recombinase alleles driven by Nos1. Alliance mouse Nos1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nos1 Mutation: 84 strains or lines available

References

• Original: J:179120 Myers MG Jr, Generation of a Nos1-cre knockin allele. MGI Direct Data Submission. 2012
• All: 42 reference(s)