Gt(ROSA)26Sor^{tm1(CAG-COP4*E123T*H134R,-tdTomato)Gfng}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-COP4*E123T*H134R,-tdTomato)Gfng}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Guoping Feng
• MGI ID: MGI:5301821
• Synonyms: R26-CAG-LSL-2XChETA-tdTomato, Rosa26-CAG-LSL-2XChETA-P2A-TdTomato-WPRE
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-COP4*E123T*H134R,-tdTomato)Gfng} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:179349
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector, which contained the CAG promoter, a loxP site flanked STOP fragment and pGK-NEO-pA cassette, 2 copies of channelrhodopsin-2 with E123T mutation (ChETA variant) and the H134R mutation, P2A-tdTomato variant of Enhanced Red Fluorescent Protein, and WPRE (Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element) sequence to enhance expression level, was inserted into the locus. (J:179349)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:179349 Feng G, Direct Data Submission 2012/01/17. MGI Direct Data Submission. 2012
• All: 12 reference(s)