Gt(ROSA)26Sor^{tm1(CAG-GFP/Eif2c2)Zjh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-GFP/Eif2c2)Zjh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Z Josh Huang
• MGI ID: MGI:5301865
• Synonyms: Gt(ROSA)26Sor^{tm1(CAG-xstpx-GFP/myc/Eif2c2)Zjh}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-GFP/Eif2c2)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:179351
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-GFP/Eif2c2)Zjh} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ago2 (MGI:2446632)

• Mutation details: A targeting vector containing loxP site flanked STOP cassette, GFP, and myc-tagged Eif2c2 coding sequence under the control of the CAG promoter was inserted in the Gt(ROSA)26Sor locus. When in the presence of Cre recombinase activity, the GFP/myc tagged Eif2c2 gene is expressed, GFP expression indicates expression of Eif2c2, and the myc epitope tag allowing affinity purification of associated miRNAs. (J:179351)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:179351 He M, et al., Cell-Type-Based Analysis of MicroRNA Profiles in the Mouse Brain. Neuron. 2012 Jan 12;73(1):35-48
• All: 6 reference(s)