Mir21a^tm1.1Mer
Targeted Allele Detail

Summary

• Symbol: Mir21a^tm1.1Mer
• Name: microRNA 21a; targeted mutation 1.1, Marc E Rothenberg
• MGI ID: MGI:5302027
• Synonyms: miR-21^-
• Gene: Mir21a Location: Chr11:86474893-86474984 bp, - strand Genetic Position: Chr11, 51.82 cM
• Alliance: Mir21a^tm1.1Mer page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:179235
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted 3' of exon 12 of the Vmp1 gene and 5' of the Mir21 sequence. Mir21 was then followed by an FRT flanked PGK-puro/deltaTK selection cassette and a second loxP site. Mir21 and the selection cassette were then excised by crossing properly targeted mice to Tg(EIIa-cre)C5379Lmgd mice. Northern blot and PCR analysis confirmed the absence of MIR21. (J:179235)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir21a Mutation: 8 strains or lines available

References

• Original: J:179235 Lu TX, et al., MicroRNA-21 limits in vivo immune response-mediated activation of the IL-12/IFN-gamma pathway, Th1 polarization, and the severity of delayed-type hypersensitivity. J Immunol. 2011 Sep 15;187(6):3362-73
• All: 5 reference(s)