Usp2^tm1.1Bjc
Targeted Allele Detail

Summary

• Symbol: Usp2^tm1.1Bjc
• Name: ubiquitin specific peptidase 2; targeted mutation 1.1, Joseph C Besharse
• MGI ID: MGI:5302030
• Gene: Usp2 Location: Chr9:43978318-44006924 bp, + strand Genetic Position: Chr9, 24.53 cM, cytoband B
• Alliance: Usp2^tm1.1Bjc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177916
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A floxed neo cassette was inserted upstream of exon 3 and a loxP site was inserted downstream of exon 4 via homologous recombination. Cre mediated recombination removed the neo cassette and exons 3 - 4. Western blot analysis of total retina lysate from homozygous mice confirmed the absence of the 69 kDa isoform but did detect low levels of expression at about 45 kDa. RT_PCR analysis also detected low levels of expression of a short transcript. Sequencing determined this transcript was formed by alternative splicing of exon 1 to exon 5. (J:177916)

Generation of the Usp2^tm1Bjc and Usp2^tm1.1Bjc alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Usp2 Mutation: 34 strains or lines available

References

• Original: J:177916 Scoma HD, et al., The de-ubiquitinylating enzyme, USP2, is associated with the circadian clockwork and regulates its sensitivity to light. PLoS One. 2011;6(9):e25382
• All: 1 reference(s)