Nkx2-2^tm3Suss
Targeted Allele Detail

Summary

• Symbol: Nkx2-2^tm3Suss
• Name: NK2 homeobox 2; targeted mutation 3, Lori Sussel
• MGI ID: MGI:5302422
• Synonyms: Nkx2.2^LacZ
• Gene: Nkx2-2 Location: Chr2:147019466-147036163 bp, - strand Genetic Position: Chr2, 72.63 cM, cytoband H
• Alliance: Nkx2-2^tm3Suss page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:178173
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, RMCE-ready, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Recombination mediated cassette exchange (RMCE) into Nkx2-2^tm1Suss inserted a full length lacZ gene with a nuclear localization signal in place of the existing cassette. Exons 1 and 2 were deleted in the original allele. LacZ expression recapitulates endogenous gene expression. (J:178173, J:187468)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nkx2-2 Mutation: 14 strains or lines available

References

• Original: J:178173 Mastracci TL, et al., Nkx2.2 and Arx genetically interact to regulate pancreatic endocrine cell development and endocrine hormone expression. Dev Biol. 2011 Nov 1;359(1):1-11
• All: 5 reference(s)