Ccn6^tm1(cre)Mawa
Targeted Allele Detail

Summary

• Symbol: Ccn6^tm1(cre)Mawa
• Name: cellular communication network factor 6; targeted mutation 1, Matthew L Warman
• MGI ID: MGI:5302829
• Synonyms: Wisp3^GFP-Cre, Wisp3^{tm1(EGFP/cre)Mawa}
• Gene: Ccn6 Location: Chr10:39026966-39039790 bp, - strand Genetic Position: Chr10, 20.19 cM
• Alliance: Ccn6^tm1(cre)Mawa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:179652
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Ccn6^tm1(cre)Mawa expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ccn6 (MGI:2685581)

• Mutation details: An EGFP-IRES-cre-construct was introduced to the first exon of Wisp3, retaining the gene's ATG translation initiation site. No full-length Wisp3 transcripts can be detected by RT-PCR. Donating investigator indicates that expression of EGFP (enhanced green fluorescence protein) cannot be visualized by fluorescence microscopy. Hetero- and homozygous mice show no apparent phenotype. When males mutants are crossed with animals carrying a conditional (floxed) allele, cre-mediated recombination is observed in 100% of offspring. (Females occasionally also express cre in germ cells but at the precise stage is unclear. Cre-mediated recombination is observed in 7% of offspring from females.)There is little or no expression of cre in most other tissues examined, including muscle, kidney, bone, liver, and lung. Occasional recombination in brain, cartilage, and heart is seen. (J:179652, J:201718)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ccn6 (mouse) Summary of all recombinase alleles driven by Ccn6. Alliance mouse Ccn6 gene page

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ccn6 Mutation: 23 strains or lines available

References

• Original: J:179652 Warman ML, Generation of a knock-in cre recombinase allele of Wisp3. MGI Direct Data Submission. 2012
• All: 3 reference(s)