Hprt1^{tm216(Ple171-EGFP/cre)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm216(Ple171-EGFP/cre)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 216, Elizabeth M Simpson
• MGI ID: MGI:5303303
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6

Mutation origin

• Mutant Cell Lines: mEMS1301, mEMS1306, mEMS1316, mEMS1329
• Germline Transmission: Unknown
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion Vector Type: Not Applicable
• Hprt1^{tm216(Ple171-EGFP/cre)Ems} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  RAMP3 (10268)

  Ramp3

  (MGI:1860292)

• Mutation details: The Ple171-EGFP/Cre plasmid (pEMS1106) was created by inserting the Ple171 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human receptor (G protein-coupled) activity modifying protein 3 (RAMP3) gene) into the multiple cloning site of the pEMS1302 plasmid vector backbone. This placed the Ple171 MiniPromoter upstream of a minimal F5 mutant-frt site, a Kozak consensus sequence, an EGFP/Cre fusion gene (enhanced green fluorescent protein (EGFP; with mutated TAA to TTA stop) and Cre recombinase gene), a nuclear localization signal, a minimal wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple171-EGFP/Cre construct was targeted as a single copy knock-in to the Hprt^b-m3 mutant locus on the X Chromosome via electroporation into E14TG2a embryonic stem cells. (J:164356)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	RAMP3 (human) Summary of all recombinase alleles driven by RAMP3. Alliance human RAMP3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94
• All: 1 reference(s)