Hprt1^{tm246(Ple194-cre/EGFP)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm246(Ple194-cre/EGFP)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 246, Elizabeth M Simpson
• MGI ID: MGI:5303336
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6

Mutation origin

• Mutant Cell Lines: mEMS559, mEMS560, mEMS561, mEMS577
• Germline Transmission: Unknown
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion Vector Type: Not Applicable
• Hprt1^{tm246(Ple194-cre/EGFP)Ems} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  SLC6A3 (6531)

  Slc6a3

  (MGI:94862)

• Mutation details: The Ple194-Cre/EGFP plasmid (pEMS1070) was created by inserting the Ple194 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (SLC6A3) gene) into the multiple cloning site of the pEMS1301 plasmid vector backbone. This placed the Ple194 MiniPromoter upstream of a minimal wildtype frt site, a Cre/EGFP fusion gene (Cre recombinase gene and enhanced green fluorescent protein (EGFP; with mutated TAA toTTA stop)), a nuclear localization signal, an SV40 early polyA signal, a second minimal wildtype frt site , a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. Of note, the sequences between the two frt sites are in reverse orientation compared to the HPRT sequences. This Ple194-Cre/EGFP construct was targeted as a single copy knock-in to the Hprt^b-m3 mutant locus on the X Chromosome via electroporation into E14TG2a embryonic stem cells. (J:164356)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	SLC6A3 (human) Summary of all recombinase alleles driven by SLC6A3. Alliance human SLC6A3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94
• All: 1 reference(s)