Rr175^tm1.1Mema
Targeted Allele Detail

Summary

• Symbol: Rr175^tm1.1Mema
• Name: regulatory region 175; targeted mutation 1.1, Masatsugu Ema
• MGI ID: MGI:5304851
• Synonyms: Flk1-DMME KO, Kdr^tm1.1Mema
• Gene: Rr175 Location: Chr5:76164984-76165733 bp Genetic Position: Chr5, Syntenic
• Alliance: Rr175^tm1.1Mema page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:178675
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph, Modified regulatory region)
• Mutation: Intragenic deletion
• Mutation details: A LoxP and FRT ste flanked neomycin resistance gene cassette replaced the Distal Multipotent Mesodermal Enhancer (DMME), located 27 kb upstream of the TSS of the Kdr gene, via homologous recombination. Cre-mediated recombination removed the neo cassette. Quantitative RT-PCR and immunohistochemical analyses indicate that expression is reduced at E7.5 in homozygous embryos. (J:178675)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr175 Mutation: 0 strains or lines available

References

• Original: J:178675 Ishitobi H, et al., Molecular basis for Flk1 expression in hemato-cardiovascular progenitors in the mouse. Development. 2011 Dec;138(24):5357-68
• All: 3 reference(s)