Notch2^{tm1.1(cre/ERT2)Sat}
Targeted Allele Detail

Summary

• Symbol: Notch2^{tm1.1(cre/ERT2)Sat}
• Name: notch 2; targeted mutation 1.1, Spyros Artavanis-Tsakonas
• MGI ID: MGI:5304913
• Synonyms: N2-CreERT2^SAT
• Gene: Notch2 Location: Chr3:97920854-98057683 bp, + strand Genetic Position: Chr3, 42.42 cM
• Alliance: Notch2^{tm1.1(cre/ERT2)Sat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:179632
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intergenic deletion
• Notch2^{tm1.1(cre/ERT2)Sat} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Notch2 (MGI:97364)

• Mutation details: A targeting vector was constructed to insert the cre/ERT2 coding sequence, a polyadenylation signal and an Frt-flanked neo selection cassette in-frame with the start codon of Notch 2, by homologous recombination in ES cells. This resulted in deletion of most of exon 1. Chimeric males were bred to Flpe-expressing females to remove the selection cassette. Mice homozygous for this allele die embryonically. (J:179632)

Generation of the various Notch knock-in mice

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Notch2 (mouse) Summary of all recombinase alleles driven by Notch2. Alliance mouse Notch2 gene page

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Notch2 Mutation: 99 strains or lines available

References

• Original: J:179632 Fre S, et al., Notch lineages and activity in intestinal stem cells determined by a new set of knock-in mice. PLoS One. 2011;6(10):e25785
• All: 4 reference(s)