Igk-J^tm1.1Lave
Targeted Allele Detail

Summary

• Symbol: Igk-J^tm1.1Lave
• Name: immunoglobulin kappa chain, joining region; targeted mutation 1.1, Laurent Verkoczy
• MGI ID: MGI:5305129
• Synonyms: 2F5, 2F5 VL⁺
• Gene: Igk-J Location: unknown Genetic Position: Chr6, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:179317
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intergenic deletion
• Mutation details: The J1, J2, and J3 regions were replaced with a rearranged human light chain sequence of the HIV-1 broadly neutralizing antibody (bnAb) 2F5 and a floxed neo cassette. Cre-mediated recombination removed the neo cassette. (J:179317)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igk-J Mutation: 11 strains or lines available

References

• Original: J:179317 Verkoczy L, et al., Rescue of HIV-1 broad neutralizing antibody-expressing B cells in 2F5 VH x VL knockin mice reveals multiple tolerance controls. J Immunol. 2011 Oct 1;187(7):3785-97
• All: 4 reference(s)