Irak1^tm1.1Pcoh
Targeted Allele Detail

Summary

• Symbol: Irak1^tm1.1Pcoh
• Name: interleukin-1 receptor-associated kinase 1; targeted mutation 1.1, Philip Cohen
• MGI ID: MGI:5305277
• Synonyms: IRAK1[D358A], IRAK1[D359A]
• Gene: Irak1 Location: ChrX:73057520-73067524 bp, - strand Genetic Position: ChrX, 37.61 cM
• Alliance: Irak1^tm1.1Pcoh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:179219
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: An F3-flanked puro cassette with a 5' loxP site was inserted upstream of a modified exon 9 in which nucleotide substitution results in the amino acid substitution of alanine for aspartic acid at position 359 (D359A). This amino acid substitution corresponds to position 358 in the human sequence. An FRT-flanked neo/IRES cassette with a 3' loxP site was inserted downstream of exon 10. Flp-mediated recombination removed the puro and neo/IRES cassette. (J:179219)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Irak1 Mutation: 16 strains or lines available

References

• Original: J:179219 Goh ET, et al., Identification of the protein kinases that activate the E3 ubiquitin ligase Pellino 1 in the innate immune system. Biochem J. 2012 Jan 1;441(1):339-46
• All: 3 reference(s)