Del(1Sdccag8-Akt3)CA1C2Ove
Transposon induced Allele Detail

Summary

• Symbol: Del(1Sdccag8-Akt3)CA1C2Ove
• Name: deletion, Chr 1, Paul A Overbeek CA1C2
• MGI ID: MGI:5306360
• Synonyms: DelCA1C2Ove, OVE2161B-CA1C-2, Sdccag8^SBT, Sdccag8^{Tn(sb-Tyr)2161B.CA1C2Ove}
• Gene: Del(1Sdccag8-Akt3)CA1C2Ove Location: unknown Genetic Position: Chr1, Syntenic

Mutation origin

• Strain of Origin: FVB/N

Mutation description

• Allele Type: Transposon induced (Null/knockout)
• Mutations: Insertion, Intergenic deletion, Intragenic deletion
• Del(1Sdccag8-Akt3)CA1C2Ove involves 2 genes/genome features (Sdccag8, Akt3) View all
• Mutation details: A Sleeping Beauty (SB) transposon transgenic approach was used to generate these mice. The pT2-BART3 transposon vector used here has (from 5' to 3') an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site [280 bp]), an AD2 splice acceptor and polyA sequence, the tyrosinase minigene TyBS, a loxP site, a polyA sequence, a Hox9c splice acceptor, and right IR/DR (280 bp). The IR/DR sequences are outward-facing. The 4.1 kb tyrosinase minigene (TyBS) contains the tyrosinase upstream regulatory sequences (2.1 kbp from the BALB/c tyrosinase promoter and the first 65 bp of exon 1), followed by the C57BL/6-derived Tyr^s-J cDNA sequence (1.9 kbp including cysteine at amino acid 103, glycine at amino acid 346, and the stop codon with polyA signal). Mice were bred to mice with widespread expression of the SB transposase (CAGGS-SB10 transgenic mice on an FVB/N genetic background). The resulting double transgenic male offspring ("seed males") have the ability to mobilize transposons in their germline; and mobilization of the outward-facing, IR/DR site-flanked transposon results in transposon integration at new sites in the genome (as well as genomic rearrangements including deletions, inversions and translocations). In line OVE2161B.CA1C2, the gene trap cassette inserts between exons 12-13 (178,833,225 bp; NCB137/mm9; L:SV40:178,833,225(+)) and between exons 1 and 2 of the Akt3 gene resulting in a deletion of a 200-250 bp fragment that encompasses exons 13-18 of Sdccag8 and exons 2-13 of Akt3. The absence of transcript expression in both genes was confirmed. (J:180503, J:258011)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Del(1Sdccag8-Akt3)CA1C2Ove Mutation: 1 strain or line available

References

• Original: J:180503 Overbeek PA, Direct Data Submission 2012/02/16. MGI Direct Data Submission. 2012
• All: 3 reference(s)