Tg(ACTB-eGFP,-RAMP1)#Afru
Transgene Detail

Summary

• Symbol: Tg(ACTB-eGFP,-RAMP1)#Afru
• Name: transgene insertion, Andrew F Russo
• MGI ID: MGI:5306398
• Synonyms: GFP-hRAMP1, Tg(ACTB-eGFP,-RAMP)#Afru
• Transgene: Tg(ACTB-eGFP,-RAMP1)#Afru Location: unknown
• Alliance: Tg(ACTB-eGFP,-RAMP1)#Afru page

Transgene origin

• Strain of Origin: C57BL/6J x SJL/J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(ACTB-eGFP,-RAMP1)#Afru expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  RAMP1 (10267)

  Ramp1

  (MGI:1858418)

• Mutation details: This transgene consists of human RAMP1 cDNA under the control of the CX1 promoter, which is a hybrid of the chicken beta-actin promoter and CMV enhancer. The CX1 vector into which RAMP1 cDNA was inserted also contains an eGFP gene flanked by loxP sites following the promoter (and upstream of RAMP1) and a beta-actin intron and bovine globin polyadenylation signal. CX1 drives ubiquitous expression of GFP, but not RAMP1 due to a translation stop codon and a polyadenylation signal in the GFP cDNA. Not until cre-mediated excision of the GFP-stop sequence at the flanking loxP sites occurs, that RAMP1 is expressed. (J:120077)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Phenotypic Similarity to Human Syndrome: migraine, susceptibility to (J:179493)

References

• Original: J:120077 Zhang Z, et al., Sensitization of calcitonin gene-related peptide receptors by receptor activity-modifying protein-1 in the trigeminal ganglion. J Neurosci. 2007 Mar 7;27(10):2693-703
• All: 6 reference(s)