Sirt1^tm2.1Mcby
Targeted Allele Detail

Summary

• Symbol: Sirt1^tm2.1Mcby
• Name: sirtuin 1; targeted mutation 2.1, Michael W McBurney
• MGI ID: MGI:5306956
• Synonyms: sirt1^Y
• Gene: Sirt1 Location: Chr10:63154784-63174814 bp, - strand Genetic Position: Chr10, 32.57 cM
• Alliance: Sirt1^tm2.1Mcby page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:180628
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Hypomorph, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A C to T mutation resulting in a histidine to tyrosine substitution at codon 355 (H355Y) was introduced in exon 5 and a floxed hygromycin/thymidine kinase fusion gene with splice acceptor and donor sequences was inserted upstream of exon 5 via homologous recombination. Cre mediated recombination removed the hygromycin/thymidine kinase fusion gene. The mutant protein is expressed at levels similar to the wild-type protein. The mutant protein lacks acetylation activity in MEFs. (J:180628)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sirt1 Mutation: 81 strains or lines available

References

• Original: J:180628 Seifert EL, et al., SirT1 catalytic activity is required for male fertility and metabolic homeostasis in mice. FASEB J. 2012 Feb;26(2):555-66
• All: 10 reference(s)