Gt(ROSA)26Sor^tm8.1Sia
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm8.1Sia
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 8.1, Shinichi Aizawa
• MGI ID: MGI:5308579
• Synonyms: Gt(ROSA)26Sor^{tm8.1(LYN/Venus)Sia}, R26-Lyn-Venus
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm8.1Sia page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:180918
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

Allele Type: Targeted (Reporter) Mutation: Insertion Mutation details: A vector, which contains (5' to 3') a splice acceptor, floxed neo/stop cassette and the N-terminal 10 amino acids containing myristoylation and palmitoylation sequences of the membrane-localized human LYN fused to the Venus reporter, was inserted into the locus. Cre-mediated recombination removed the neo cassette. (J:180918)

Conditional targeting of the ROSA26 locus to generate various reporter lines

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:180918 Abe T, et al., Establishment of conditional reporter mouse lines at ROSA26 locus for live cell imaging. Genesis. 2011 Jul;49(7):579-90
• All: 2 reference(s)