Mcph1^tm1.1Zqw
Targeted Allele Detail

Summary

• Symbol: Mcph1^tm1.1Zqw
• Name: microcephaly, primary autosomal recessive 1; targeted mutation 1.1, Zhao-Qi Wang
• MGI ID: MGI:5308745
• Synonyms: Mcph1^delta
• Gene: Mcph1 Location: Chr8:18645147-18853205 bp, + strand Genetic Position: Chr8, 10.24 cM, cytoband A3
• Alliance: Mcph1^tm1.1Zqw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:180706
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neo cassette was inserted upstream of exon 4. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 5. Cre-mediated recombination removed exons 4 and 5 as well as the neo cassette. Western blot analysis confirmed the absence of protein expression in primary mouse embryonic fibroblasts and the brain. (J:180706)

Expression

In Mice Carrying this Mutation:	9 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcph1 Mutation: 58 strains or lines available

References

• Original: J:180706 Gruber R, et al., MCPH1 regulates the neuroprogenitor division mode by coupling the centrosomal cycle with mitotic entry through the Chk1-Cdc25 pathway. Nat Cell Biol. 2011 Nov;13(11):1325-34
• All: 7 reference(s)