Mirc21^tm1.1Lhe
Targeted Allele Detail

Summary

• Symbol: Mirc21^tm1.1Lhe
• Name: microRNA cluster 21, including Mir34c through Mir34b; targeted mutation 1.1, Lin He
• MGI ID: MGI:5308794
• Synonyms: miR-34bc^-, Mir34b/Mir34c^tm1.1Ghan, Mirc21^tm1.1Ghan
• Gene: Mirc21 Location: Chr9:51014334-51014945 bp Genetic Position: Chr9, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:180692
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of Mir34b and an FRT-flanked neo cassette with a 3' loxP site was inserted downstream of Mir34c. Cre-mediated recombination removed the neo cassette and both microRNAs. Real-time PCR confirmed the absence of transcript expression in mouse embryonic fibroblasts. (J:180692)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mirc21 Mutation: 5 strains or lines available

References

• Original: J:180692 Choi YJ, et al., miR-34 miRNAs provide a barrier for somatic cell reprogramming. Nat Cell Biol. 2011 Nov;13(11):1353-60
• All: 7 reference(s)