Tg(CD2-NPM/ALK)4Sudt
Transgene Detail

Summary

• Symbol: Tg(CD2-NPM/ALK)4Sudt
• Name: transgene insertion 4, Suzanne Turner
• MGI ID: MGI:5310806
• Synonyms: CD2-NPM-ALK, CD2/NPM-ALK, NPM-ALK, Tg(CD2-NPM/ALK)1Sudt
• Transgene: Tg(CD2-NPM/ALK)4Sudt Location: unknown
• Alliance: Tg(CD2-NPM/ALK)4Sudt page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: This transgene contains a cDNA encoding the human tumor-associated NPM1/ALK fusion protein under control of the regulatory sequences of the human CD2 gene. The chimeric tyrosine kinase, comprising the N-terminal 40% of the ubiquitously expressed protein nucleophosmin (nucleolar phosphoprotein B23, numatrin) (NPM1) joined to the entire cytoplasmic tail of anaplastic lymphoma kinase (ALK), is expressed in human tumors from a fusion gene resulting from the t(2;5)(p23;q35) chromosomal translocation that is present in 40-50% of anaplastic large cell lymphomas. The NPM1/ALK cDNA was inserted into a CD2 expression cassette that includes 5 kb of 5' flanking sequence containing the promoter, transcription initiation site and first intron; and 5.5 kb of 3' flanking sequence containing the two polyadenylation signals and locus control region (LCR) of the human CD2 gene. Expression of the mRNA is detected by reverse transcription/polymerase chain reaction (RT-PCR) analysis and of the chimeric protein by western blot analysis only in tumors, and not in pre-cancerous thymocytes, of transgenic animals. (J:105228, J:181238)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tg(CD2-NPM/ALK)4Sudt

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:181238 Turner SD, et al., CD2 promoter regulated nucleophosmin-anaplastic lymphoma kinase in transgenic mice causes B lymphoid malignancy. Anticancer Res. 2006 Sep-Oct;26(5A):3275-9
• All: 2 reference(s)