Ift140^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ift140^{tm1c(KOMP)Wtsi}
• Name: intraflagellar transport 140; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5311828
• Synonyms: Ift140^flox
• Gene: Ift140 Location: Chr17:25235056-25318461 bp, + strand Genetic Position: Chr17, 12.53 cM
• Alliance: Ift140^{tm1c(KOMP)Wtsi} page
• IMPC: Ift140 gene page

Mutation origin

• Mutant Cell Line: EPD0073_5_F01
• Germline Transmission: Earliest citation of germline transmission: J:181533
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 25168608 of Chromosome 17 upstream of the critical exon(s) (Build 37). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 7 at position 25169359. Exon 7 is thus flanked by loxP sites. Expression of flp recombinase excised the lacZ reporter sequence and the neomycin selection cassette leaving exon 7 flanked by loxP sites. (J:148605, J:173534, J:181533)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ift140 Mutation: 71 strains or lines available

References

• Original: J:181533 Jonassen JA, et al., Disruption of IFT complex A causes cystic kidneys without mitotic spindle misorientation. J Am Soc Nephrol. 2012 Apr;23(4):641-51
• All: 5 reference(s)