Tg(Wap-TAg)12Gmn
Transgene Detail

Summary

• Symbol: Tg(Wap-TAg)12Gmn
• Name: transgene insertion 12, A Graessmann
• MGI ID: MGI:5312328
• Synonyms: WAP-SVT, WAP-SVT/12
• Transgene: Tg(Wap-TAg)12Gmn Location: unknown
• Alliance: Tg(Wap-TAg)12Gmn page

Transgene origin

• Strain of Origin: NMRI

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The transgene consists of a 1600 bp DNA fragment containing the promoter of the mouse whey acidic protein gene joined to the SV40 early region, which includes the overlapping sequences encoding the SV40 large and small T-antigen genes. So that only the large T antigen is expressed, the 5' splice site of the small t antigen mRNA was modified by site directed mutagenesis (AAG/GTAAAT to AAG/CGCAAT) to prevent splicing of the small t transcript. (J:56330, J:69711)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Three transgenic mouse lines bearing this construct, which the authors designate WAP-SVT/12, WAP-SVT/14 and WAP-SVT/17, are described. Most data shown are for mice of Line 12. (J:69711)

References

• Original: J:69711 Goetz F, et al., The SV40 small t-antigen prevents mammary gland differentiation and induces breast cancer formation in transgenic mice; truncated large T-antigen molecules harboring the intact p53 and pRb binding region do not have this effect. Oncogene. 2001 Apr 26;20(18):2325-32
• All: 2 reference(s)