Tg(Avil-icre/ERT2)AJwo
Transgene Detail

Summary

• Symbol: Tg(Avil-icre/ERT2)AJwo
• Name: transgene insertion A, John N Wood
• MGI ID: MGI:5312922
• Synonyms: AvCreERT2, Tg(Avil-cre/ERT2)AJwo
• Transgene: Tg(Avil-icre/ERT2)AJwo Location: Chr6:62253083-62615557 bp Genetic Position: Chr6, Syntenic
• Alliance: Tg(Avil-icre/ERT2)AJwo page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Avil-icre/ERT2)AJwo involves 1 genes/genome features (Ccser1) View all
• Tg(Avil-icre/ERT2)AJwo expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Avil (MGI:1333798)

• Mutation details: BAC recombineering was used to generate a vector with the cre/ERT2 sequence fused with the start codon in exon 2 of a partial advillin gene sequence located in BAC clone RPCI23-424F19. An Frt-flanked kanamycin selection marker was included in the initial cre cassette and was subsequently excised by induction of Flp recombinase in EL250 cells. The purified construct was used for pronuclear injection to generate 8 founder lines. Cre activity was verified in offspring of founders by breeding to ROSA26-lacZ reporter mice. Founder A exhibited strongest staining in DRGs and was analyzed in detail. Line A carries about 10 transgene copies (estimated by Southern blotting.) The transgene has integrated into the intronic region between exons 10 and 11 of the Ccser1 gene on chromosome 6 (chr6: 62276099-62638573). The insertion creates an 82 bp deletion. (J:181671)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Avil (mouse) Summary of all recombinase alleles driven by Avil. Alliance mouse Avil gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:181671 Lau J, et al., Temporal control of gene deletion in sensory ganglia using a tamoxifen-inducible Advillin-Cre-ERT2 recombinase mouse. Mol Pain. 2011;7:100
• All: 34 reference(s)