Cigs2^tm1.1Anse
Targeted Allele Detail

Summary

• Symbol: Cigs2^tm1.1Anse
• Name: conserved intergenic sequence 2; targeted mutation 1.1, K Mark Ansel
• MGI ID: MGI:5313809
• Synonyms: deltaV, HS V^-
• Gene: Cigs2 Location: unknown Genetic Position: Chr11, Syntenic
• Alliance: Cigs2^tm1.1Anse page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:181313
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: A loxP site flanked neomycin resistance gene cassette replaced the 810 bp encompassing the DNaseI hypersensitivity site 5 (HS5) in conserved non-coding sequence 2 (CNS-2) downstream of Il4, between it and Kif3a. Cre-mediated recombination removed the neo cassette. RT-PCR confirmed the reduction of Il4 and Il13 transcript expression in Th2 polarized CD4+ T cells. (J:181313)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cigs2 Mutation: 0 strains or lines available

References

• Original: J:181313 Vijayanand P, et al., Interleukin-4 Production by Follicular Helper T Cells Requires the Conserved Il4 Enhancer Hypersensitivity Site V. Immunity. 2012 Feb 24;36(2):175-187
• All: 1 reference(s)