Tg(Krt8-cre/ERT2,-GFP)1Xin
Transgene Detail

Summary

• Symbol: Tg(Krt8-cre/ERT2,-GFP)1Xin
• Name: transgene insertion 1, Li Xin
• MGI ID: MGI:5314229
• Synonyms: K8-CreER^T2, Tg(Krt8-cre/ERT2)1Xin
• Transgene: Tg(Krt8-cre/ERT2,-GFP)1Xin Location: unknown
• Alliance: Tg(Krt8-cre/ERT2,-GFP)1Xin page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Krt8-cre/ERT2,-GFP)1Xin expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Krt8 (MGI:96705)

• Mutation details: A BAC clone (RP23-234D2) was modified by recombineering methods to insert the cre/ERT2 fusion sequence at the translational start codon of Krt8. An IRES-GFP expression cassette was inserted downstream of the cre sequence. The linearized BAC construct was microinjected into fertilized C57BL/6J oocytes. RT-PCR was used to screen founder mice and one line was chosen for characterization based on its cre/ERT2 expression pattern which closely recapitulated endogenous Krt8 expression. This line carries 2 copies of the transgene (based on quantitative PCR performed on genomic DNA) with a single insertion site. However, although GFP is detectable by RT-PCR and Western blot analysis, GFP fluorescence cannot be visualized by fluorescent microscopy in tissue sections or by FACS analysis of dissociated prostate cells. (J:181459, J:181829)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Krt8 (mouse) Summary of all recombinase alleles driven by Krt8. Comparative matrix view of recombinase activities Alliance mouse Krt8 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:181459 Choi N, et al., Adult Murine Prostate Basal and Luminal Cells Are Self-Sustained Lineages that Can Both Serve as Targets for Prostate Cancer Initiation. Cancer Cell. 2012 Feb 14;21(2):253-65
• All: 11 reference(s)