Tg(EEF1A1-Socs6)1Pwg
Transgene Detail

Summary

• Symbol: Tg(EEF1A1-Socs6)1Pwg
• Name: transgene insertion 1, Ping Wang
• MGI ID: MGI:5314513
• Synonyms: SOCS-6 transgenic line 1, Tg1
• Transgene: Tg(EEF1A1-Socs6)1Pwg Location: unknown
• Alliance: Tg(EEF1A1-Socs6)1Pwg page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:181312
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(EEF1A1-Socs6)1Pwg expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Socs6 (MGI:1924885)

• Mutation details: A full length mouse Sox6 cDNA modified to append a human MYC tag to the carboxyl terminus of the protein product was cloned into the pEFneo vector downstream of the human eukaryotic elongation factor 1 alpha 1 promoter. Northern blot analysis using a Socs6 cDNA probe and western blot analysis with an anti-MYC antibody demonstrate that mice of line 1 express transgene-derived mRNA and protein, respectively, at 5-fold the levels expressed by wild-type mice from the endogenous gene in liver, muscle and T-lymphocytes. (J:181312)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

This is one of two transgenic lines that were generated and characterized. The other, Tg(EEF1A1-Socs6)2Pwg, expresses the transgene at 10-fold the level at which wild-type mice express the endogenous gene. (J:181312)

References

• Original: J:181312 Li L, et al., Insulin induces SOCS-6 expression and its binding to the p85 monomer of phosphoinositide 3-kinase, resulting in improvement in glucose metabolism. J Biol Chem. 2004 Aug 13;279(33):34107-14
• All: 2 reference(s)