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Tg(EEF1A1-Socs6)2Pwg
Transgene Detail
Summary
Symbol: Tg(EEF1A1-Socs6)2Pwg
Name: transgene insertion 2, Ping Wang
MGI ID: MGI:5314516
Synonyms: SOCS-6 transgenic line 2, Tg2
Transgene: Tg(EEF1A1-Socs6)2Pwg  Location: unknown  
Alliance: Tg(EEF1A1-Socs6)2Pwg page
Transgene
origin
Germline Transmission:  Earliest citation of germline transmission: J:181312
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(EEF1A1-Socs6)2Pwg expresses 1 gene
 
Mutation detailsA full length mouse Sox6 cDNA modified to append a human MYC tag to the carboxyl terminus of the protein product was cloned into the pEFneo vector downstream of the human eukaryotic elongation factor 1 alpha 1 promoter. Northern blot analysis using a Socs6 cDNA probe and western blot analysis with an anti-MYC antibody demonstrate that mice of line 2 express transgene-derived mRNA and protein, respectively, at 10-fold the levels expressed by wild-type mice from the endogenous gene in liver, muscle and T-lymphocytes. (J:181312)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Notes
This is one of two transgenic lines that were generated and characterized. The other, Tg(EEF1A1-Socs6)1Pwg, expresses the transgene at 5-fold the level at which wild-type mice express the endogenous gene. (J:181312)
References
Original:  J:181312 Li L, et al., Insulin induces SOCS-6 expression and its binding to the p85 monomer of phosphoinositide 3-kinase, resulting in improvement in glucose metabolism. J Biol Chem. 2004 Aug 13;279(33):34107-14
All:  2 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory