Car5b^tm1Sly
Targeted Allele Detail

Summary

• Symbol: Car5b^tm1Sly
• Name: carbonic anhydrase 5b, mitochondrial; targeted mutation 1, William S Sly
• MGI ID: MGI:5314752
• Gene: Car5b Location: ChrX:162759818-162810993 bp, - strand Genetic Position: ChrX, 76.03 cM, cytoband F5
• Alliance: Car5b^tm1Sly page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:181790
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Thirty bp (codons for 10 amino acids) in exon 3 were replaced with a floxed cassette composed of a neomycin resistance gene and cre placed under the direction of a testis specific angiotensin converting enzyme pomoter. Cre expression in the germline of chimeric male mice excised the inserted cassette leaving a deletion in exon 3 including two of three zinc binding histidine residues and introducing a frameshift beginning in exon 4. A stop codon was created after 11 missence amino acids. (J:181790, J:196135)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Car5b Mutation: 15 strains or lines available

References

• Original: J:181790 Price TO, et al., Topiramate treatment protects blood-brain barrier pericytes from hyperglycemia-induced oxidative damage in diabetic mice. Endocrinology. 2012 Jan;153(1):362-72
• All: 2 reference(s)