Mir137^tm1Mtm
Targeted Allele Detail

Summary

• Symbol: Mir137^tm1Mtm
• Name: microRNA 137; targeted mutation 1, Michael McManus
• MGI ID: MGI:5314770
• Gene: Mir137 Location: Chr3:118227506-118227578 bp, + strand Genetic Position: Chr3, 51.71 cM
• Alliance: Mir137^tm1Mtm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:181752
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin resistance gene cassette, a second FRT site and a second loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and a third loxP site immediately downstream of the microRNA stem loop. This was intended to create a knockout-first conditional-ready reporter knockout allele, but no beta-galactosidase activity could be detected in adult or embryonic tissues of heterozygous mice, nor could a functional conditional-ready allele be created after flp-mediated deletion of the insert, suggesting that the inserted FRT and/or LoxP recombination sites interfere with the transcription of the allele. (J:181752, J:244465)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mir137 Mutation: 5 strains or lines available

References

• Original: J:181831 W.M. Keck Center for Noncoding RNAs, Information obtained from the W.M. Keck Center for Noncoding RNAs. Unpublished. 2012
• All: 3 reference(s)