Mir135b^tm1.1Mtm
Targeted Allele Detail

Summary

• Symbol: Mir135b^tm1.1Mtm
• Name: microRNA 135b; targeted mutation 1.1, Michael McManus
• MGI ID: MGI:5314945
• Gene: Mir135b Location: Chr1:132125826-132125922 bp, + strand Genetic Position: Chr1, 57.24 cM
• Alliance: Mir135b^tm1.1Mtm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:181752
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Cre-mediated recombination removed the neo cassette and the microRNA stem loop from Mir135b^tm1Mtm. qRT-PCR analysis confirmed the loss of the microRNA stem loop. Beta-galactosidase expression is observed in brain and spinal cord of E18.5 embryos and adult inner ear. (J:181752)

Expression

In Mice Carrying this Mutation:

6 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir135b Mutation: 3 strains or lines available

References

• Original: J:181752 Park CY, et al., A resource for the conditional ablation of microRNAs in the mouse. Cell Rep. 2012;1(4):385-91
• All: 2 reference(s)