Mir205^tm1.1Mtm
Targeted Allele Detail

Summary

• Symbol: Mir205^tm1.1Mtm
• Name: microRNA 205; targeted mutation 1.1, Michael McManus
• MGI ID: MGI:5314948
• Gene: Mir205 Location: Chr1:193189771-193189838 bp, - strand Genetic Position: Chr1, 97.82 cM
• Alliance: Mir205^tm1.1Mtm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:181752
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Mutation details: Cre-mediated recombination removed the neo cassette and the microRNA stem loop from Mir205^tm1Mtm. qRT-PCR analysis confirmed the loss of the microRNA stem loop. Beta-galactosidase expression is observed in forebrain, pharyngeal arches and limbs at E11.5, and thymus, skin and kidney of E18.5 embryos (J:181752)

Generation of the Mir205^tm1Mtm, Mir205^tm1.1Mtm, and Mir205^tm1.2Mtm alleles

Expression

In Mice Carrying this Mutation:	72 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir205 Mutation: 3 strains or lines available

References

• Original: J:181752 Park CY, et al., A resource for the conditional ablation of microRNAs in the mouse. Cell Rep. 2012;1(4):385-91
• All: 5 reference(s)