Mirlet7d^tm1.1Mtm
Targeted Allele Detail

Summary

• Symbol: Mirlet7d^tm1.1Mtm
• Name: microRNA let7d; targeted mutation 1.1, Michael McManus
• MGI ID: MGI:5314965
• Gene: Mirlet7d Location: Chr13:48689488-48689590 bp, - strand Genetic Position: Chr13, 24.84 cM
• Alliance: Mirlet7d^tm1.1Mtm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:181831
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Cre-mediated recombination removed the neo cassette and the microRNA stem loop from Mirlet7d^tm1Mtm. qRT-PCR analysis confirmed the loss of the microRNA. Beta-galactosidase expression is observed in brain and spinal cord of E11.5 embryos. (J:181752)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirlet7d Mutation: 4 strains or lines available

References

• Original: J:181831 W.M. Keck Center for Noncoding RNAs, Information obtained from the W.M. Keck Center for Noncoding RNAs. Unpublished. 2012
• All: 2 reference(s)