Mirc13^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc13^tm1Wtsi
• Name: microRNA cluster 13, including Mir141 through Mir200c; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5315148
• Synonyms: miR-141/200c^-, Mir141/Mir200c cluster 6N3 KO, Mir141^{tm1(mirKO)Wtsi}, Mirc13^{tm1(mirKO)Wtsi}
• Gene: Mirc13 Location: Chr6:124694877-124695353 bp Genetic Position: Chr6, Syntenic

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_11A1, mirKO_ES_PuDtk_11A2, mirKO_ES_PuDtk_11A3, mirKO_ES_PuDtk_11A4, mirKO_ES_PuDtk_11A5, mirKO_ES_PuDtk_11A6
• Germline Transmission: Earliest citation of germline transmission: J:174268
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: The microRNA cluster including Mir141 and Mir200c was replaced via homologous recombination with a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters. The inserted selection cassette was flanked by LoxP and F3/FRT sites. The deletion starts at position 124718404 and ends at position 124717909 of Chromosome 6 (Genome Build 38). (J:94077, J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc13 Mutation: 3 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 3 reference(s)