Tg(tetO-COX8A/PstI*)1Ctm
Transgene Detail

Summary

• Symbol: Tg(tetO-COX8A/PstI*)1Ctm
• Name: transgene insertion 1, Carlos T Moraes
• MGI ID: MGI:5316004
• Synonyms: mito-PstI
• Transgene: Tg(tetO-COX8A/PstI*)1Ctm Location: unknown
• Alliance: Tg(tetO-COX8A/PstI*)1Ctm page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-COX8A/PstI*)1Ctm expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  COX8A (1351)

  Cox8a

  (MGI:105959)

• Mutation details: This transgene consists of a mammalianized version of the bacterial PstI gene positioned behind a 5' mitochondrial targeting sequence from human COX VIII (COX8a) gene under the control of the tetracycline response element (TRE) promoter. The intervening sequence 8 (IVS8) was introduced between the TRE and the mito-PstI coding sequence. Southern blots indicate reduced levels of mtDNA and accumulation of partially deleted mtDNA in mice mated with tetracycline-controlled transactivator protein-expressing mice, indicating that the expressed protein is functional and induces double-stranded breaks in mtDNA. Treatment with Dox prevents the expression of the transgene. (J:145737)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:145737 Fukui H, et al., Mechanisms of formation and accumulation of mitochondrial DNA deletions in aging neurons. Hum Mol Genet. 2009 Mar 15;18(6):1028-36
• All: 7 reference(s)