Pik3c3^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pik3c3^{tm1c(EUCOMM)Wtsi}
• Name: phosphatidylinositol 3-kinase catalytic subunit type 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5316387
• Synonyms: Vps34^f
• Gene: Pik3c3 Location: Chr18:30405800-30481179 bp, + strand Genetic Position: Chr18, 17.41 cM
• Alliance: Pik3c3^{tm1c(EUCOMM)Wtsi} page
• IMPC: Pik3c3 gene page

Pik3c3^{tm1c(EUCOMM)Wtsi}/Pik3c3^{tm1c(EUCOMM)Wtsi} MEFs transduced with cre-expressing adenovirus exhibit large translucent vacuoles

Show the 6 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: EPD0103_4_A07
• Germline Transmission: Earliest citation of germline transmission: J:182628
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Conditional ready) Mutation: Insertion Vector: L1L2_gt2 Mutation details: An FRT-flanked promoterless cassette, which contains a splice acceptor, beta-geo and polyadenylation sequence, with a 3' loxP site was inserted upstream of exon 4. An additional loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the promoterless cassette and left exon 4 floxed. (J:182628)

Generation of the Pik3c3^tm1a(EUCOMM)Wtsi and Pik3c3^tm1c(EUCOMM)Wtsi alleles

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pik3c3 Mutation: 48 strains or lines available

Notes

Cell line EPD0103_4_A07 was used to make chimeric mice. Germline transmission was accomplished and subsequenct recombination established the allele. J:182628

References

• Original: J:182628 Jaber N, et al., Class III PI3K Vps34 plays an essential role in autophagy and in heart and liver function. Proc Natl Acad Sci U S A. 2012 Feb 7;109(6):2003-8
• All: 7 reference(s)