Col1a1^{tm1(tetO-YAP1*)Fcam}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-YAP1*)Fcam}
• Name: collagen, type I, alpha 1; targeted mutation 1, Fernando D Camargo
• MGI ID: MGI:5316453
• Synonyms: TetO-YAP1, Yap1-S127A
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-YAP1*)Fcam} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141457
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Humanized sequence, Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-YAP1*)Fcam} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  YAP1 (10413)

  Yap1	(MGI:103262)

• Mutation details: RMCE on KH2 cells inserted a cassette, which contained a tetracycline response element driving doxycycline-inducible expression of the human cDNA with nucleotide substitutions that result in the amino acid substitution of alanine for serine at position 127 (S127A), into the 3'UTR. This mutation produces a protein with increased nuclear localization and transcriptional activity compared with the wild-type protein. (J:141457)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:141457 Camargo FD, et al., YAP1 increases organ size and expands undifferentiated progenitor cells. Curr Biol. 2007 Dec 4;17(23):2054-60
• All: 38 reference(s)