Mitf^tm7.1Arnh
Targeted Allele Detail

Summary

• Symbol: Mitf^tm7.1Arnh
• Name: melanogenesis associated transcription factor; targeted mutation 7.1,
• MGI ID: MGI:5316513
• Synonyms: Mitf^{mi-S73AdeltaNeo}
• Gene: Mitf Location: Chr6:97784013-97998310 bp, + strand Genetic Position: Chr6, 45.05 cM
• Alliance: Mitf^tm7.1Arnh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:182722
• Parent Cell Line: LC3 (ES Cell)
• Strain of Origin: (C57BL/6N x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The targeted mutation was within exon 2B to change the coding sequence for amino acid 73 from serine to alanine (S73A) and a silent nucleotide change 10bp upstream to generate a diagnostic restriction site. A floxed neomycin selection cassette was also inserted into intron 2. The selection cassette was subsequently removed by cre excision. (J:182722)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mitf Mutation: 74 strains or lines available

References

• Original: J:182722 Debbache J, et al., In vivo role of alternative splicing and serine phosphorylation of the microphthalmia-associated transcription factor. Genetics. 2012 May;191(1):133-44
• All: 1 reference(s)