Piwil1^tm1.2Embrp
Targeted Allele Detail

Summary

• Symbol: Piwil1^tm1.2Embrp
• Name: piwi-like RNA-mediated gene silencing 1; targeted mutation 1.2, Ramesh Pillai
• MGI ID: MGI:5316871
• Gene: Piwil1 Location: Chr5:128813135-128832538 bp, + strand Genetic Position: Chr5, 67.86 cM
• Alliance: Piwil1^tm1.2Embrp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:182800
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Cre-mediated recombination of Piwil1^tm1.1Embrp removed exon 16 and the modified exon 17. Western blot analysis confirmed the absence of protein expression in testes extracts. (J:182800)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Piwil1 Mutation: 28 strains or lines available

References

• Original: J:182800 Reuter M, et al., Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing. Nature. 2011 Dec 8;480(7376):264-7
• All: 2 reference(s)