Sema3d^{tm2.1(GFP/cre)Joe}
Targeted Allele Detail

Summary

• Symbol: Sema3d^{tm2.1(GFP/cre)Joe}
• Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D; targeted mutation 2.1, Jonthan Epstein
• MGI ID: MGI:5317896
• Synonyms: Sema3D^GFPcre, Sema3d^{tm1.1(GFP/cre)Cjt}
• Gene: Sema3d Location: Chr5:12433352-12638915 bp, + strand Genetic Position: Chr5, 3.97 cM, cytoband A2
• Alliance: Sema3d^{tm2.1(GFP/cre)Joe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:183083
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Sema3d^{tm2.1(GFP/cre)Joe} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sema3d (MGI:1860118)

• Mutation details: An eGFP/cre fusion gene and an Frt-flanked neo cassette was inserted into the Sema3d locus at the ATG codon, replacing the remaining exon 2 sequence by homologous recombination in TL-1 ES cells. The neo was deleted by crossing germline mutants to Flp-expressing mice. (J:183083)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sema3d (mouse) Summary of all recombinase alleles driven by Sema3d. Comparative matrix view of recombinase activities Alliance mouse Sema3d gene page

Expression

In Mice Carrying this Mutation:

22 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sema3d Mutation: 53 strains or lines available

References

• Original: J:183083 Katz TC, et al., Distinct compartments of the proepicardial organ give rise to coronary vascular endothelial cells. Dev Cell. 2012 Mar 13;22(3):639-50
• All: 7 reference(s)