Gt(ROSA)26Sor^{tm1.1(CAG-COX8A/Dendra2)Dcc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-COX8A/Dendra2)Dcc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, David C Chan
• MGI ID: MGI:5318241
• Synonyms: Gt(ROSA)26Sor^{tm1.1(CAG-Mito-Dendra2)Dcc}, mito-Dendra2, PhAM^excised
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-COX8A/Dendra2)Dcc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:183305
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: Dendra2 (a green to red irreversibly photoswitchable monomeric fluorescent protein derived from octocoral Dendronephthya sp.) fused to the mitochondrial targeting signal of subunit VIII of cytochrome c oxidase (mito-Dendra2), bovine growth hormone (bGH) polyadenylation signal, and an FRT-flanked neomycin cassette were introduced to Gt(ROSA)26Sor downstream of a CAG (CMV-beta-actin) promoter and loxP-flanked termination signal. Flp-mediated recombination removed the neo cassette and cre-mediated recombination excised the floxed termination cassette. (J:183306, J:189358)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:183306 Chan DC, Direct Data Submission 2012/05/14. MGI Direct Data Submission. 2012
• All: 35 reference(s)