Slc12a6^tm1.1Garo
Targeted Allele Detail

Summary

• Symbol: Slc12a6^tm1.1Garo
• Name: solute carrier family 12, member 6; targeted mutation 1.1, Guy A Rouleau
• MGI ID: MGI:5318539
• Synonyms: Slc12a6^delta18
• Gene: Slc12a6 Location: Chr2:112096659-112193508 bp, + strand Genetic Position: Chr2, 56.99 cM
• Alliance: Slc12a6^tm1.1Garo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:183239
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT and LoxP flanked neo cassette was inserted upstream of exon 18 and a loxP site was inserted downstream of exon 18 via homologous recombination. Cre mediated recombination removed exon 18. RT-PCR analysis detected the presence of a lower molecular weight product. Western blot analysis detected a small amount of truncated protein in brain lysates from homozygous mice. (J:183239)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc12a6 Mutation: 120 strains or lines available

References

• Original: J:183239 Shekarabi M, et al., Loss of neuronal potassium/chloride cotransporter 3 (KCC3) is responsible for the degenerative phenotype in a conditional mouse model of hereditary motor and sensory neuropathy associated with agenesis of the corpus callosum. J Neurosci. 2012 Mar 14;32(11):3865-76
• All: 2 reference(s)