Rhag^tm1Goo
Targeted Allele Detail

Summary

• Symbol: Rhag^tm1Goo
• Name: Rhesus blood group-associated A glycoprotein; targeted mutation 1, Dominique Goossens
• MGI ID: MGI:5318589
• Gene: Rhag Location: Chr17:41122017-41151645 bp, + strand Genetic Position: Chr17, 19.54 cM
• Alliance: Rhag^tm1Goo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:182122
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Duplication, Insertion
• Rhag^tm1Goo involves 1 genes/genome features (Rhag) View all
• Mutation details: A vector isolated from a targeting vector library described in J:115976 was used to generate this null allele. The vector consisted of an upstream portion of the Hprt gene, a loxP site, a puromycin resistance gene, and a 7.1 kb genomic fragment, including exons 2-5, in which a deletion was created. This vector was used in gap repair targeting where the genomic gap in the vector is repaired by the chromosomal DNA template, creating a duplication on the chromosome. Western blot analysis confirmed the absence of expression in homozygous mice. (J:182122)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Rhag Mutation: 27 strains or lines available

References

• Original: J:182122 Goossens D, et al., Generation and characterisation of Rhd and Rhag null mice. Br J Haematol. 2010 Jan;148(1):161-72
• All: 2 reference(s)